New Delhi: Chief Minister Arvind Kejriwal inaugurated the Delhi government’s second laboratory for genome sequencing of the Sars-CoV-2 virus, which causes COVID-19, at the Institute of Liver and Biliary Sciences (ILBS) in South Delhi’s Vasant Kunj. First such facility closed at Lok Nayak Hospital. CM Kejriwal said, “I think the people of Delhi will benefit a lot from this facility.” He tweeted that with the help of these labs, the identity and severity of any new form of coronavirus can be detected. Delhiites will get a lot of benefit from this technology during the pandemic.
Dr. Shiv Kumar Sarin, Director, ILBS informed that ILBS preserves the RNA of positive COVID-19 samples and it will be able to sequence them in their entirety, all its 30,000 molecules, results will be available in 5-7 days. The Delhi government has approved the NovaSeq machine for ILBS which will be capable of sequencing hundreds of samples in a week. This facility of the Delhi government will also help in the classification of new strains or variants of coronavirus on the basis of genomic data. Health Minister Satyendar Jain and ILBS Director Dr Shiv Kumar Sareen along with CM Kejriwal inaugurated the launch of Genome Sequencing Facility at ILBS.
Addressing the media after his visit, CM Kejriwal said, “It is with great pride and pleasure that I announce the starting of a laboratory at ILBS for the purpose of genome sequencing to identify and analyze COVID-19 variants. I do. The lab has already started. The first set of results will come in the next 4-5 days. Yesterday, we started a similar lab at LNJP hospital, but it’s going to be even more advanced. I really think It is hoped that the people of Delhi will benefit immensely from this laboratory and the facility that it will cater to. Till now, we were dependent on NCDC and other centers of the central government for this sequencing. But now, we are independent and identify variants in a timely manner. and take the right actions to strategize our plans for any of the next waves, if they come.”
The facility will help generate state-of-the-art whole genome sequence data of SARS CoV2 virus for identification and screening of new strains and variants with clinical importance, circulating in and around the Delhi NCR region. This facility can sequence almost all 30,000 molecules of the coronavirus, in fact, the entire length of the virus. By doing this we can detect any type of mutation in any part of the virus, not just the spike protein region. The ILBS facility is equipped with the latest machines called NextSec and MiSeq. These centers can sequence the entire virus according to 400 sequences in a week. The Delhi government has approved a new machine for ILBS, NovaSeq, which will allow it to sequence hundreds of viral sequences every week. These facilities will also help in classification of new strains or variants of coronavirus based on genomic data.
Announcing this further on Twitter, CM Kejriwal said, ‘Keeping in view the future preparations, today Delhi Government’s second genome sequencing facility has been started in ILBS. Any new form of coronavirus can be traced. Delhiites will get a lot of benefit from this technology during the pandemic.”
Dr. Shiv Kumar Sareen, Director, ILBS Hospital, elaborated on the process and said, “After the RTPCR test is done, we preserve the RNA and sequence their virus. Let’s say 100 people are COVID positive. found, we will be able to sequence five of those tests as a whole, all 30,000 molecules. After sequencing, we will be able to identify through the results whether the variant is new or existing. It is not that each variant Extremely alarming, but we should be well informed about the new cases coming to Delhi and their types. We already have more than 1,00,000 samples from last months and on the request of Health Minister or Chief Minister, we We can sequence a portion of them as well. We can sequence more than 300 samples in a week and their results can be made available within 5 to 7 days.”
Dr. Sareen further added that ILBS has one of the most modern equipment, accessories, processing laboratories and quality control practices to undertake an effort of this magnitude. He added that ILBS is also part of the national network INSACOG, and maintains national standards.
RT-PCR positive samples collected in the form of nasopharyngeal and oropharyngeal swabs from different districts of Delhi will be taken to ILBS and genome sequencing for this will be done in a time bound manner and the report will be sent to IDSP Delhi on regular basis. Continuous virological surveillance by genome sequencing of SARS CoV2 will help in controlling the circulating viral strain as well as timely detection and detection of any new variants in the city and prevent any wave of COVID-19 infection in Delhi. will be prepared to deal with it. in a better way. For the same purpose Delhi government is going to start genome sequencing for SARS CoV2 in ILBS.
The COVID RT-PCR laboratory at the Institute of Liver and Biliary Sciences was the first ICMR certified Delhi government laboratory to start RT-PCR testing in Delhi. Till date, around 2.5 lakh samples have been tested for SARS CoV-2 in ILBS with around 13% positivity rate.
As the world grapples with one of the largest health pandemics ever seen, causing social and economic disruption on a global scale, genomic sequencing remains a fundamental tool for understanding how viruses evolve and How our defenses against this need to be optimized. It is a technology that helps to understand and identify changing viral strains of common human viruses. Genome sequencing of SARS CoV2 will help identify strains that are more life-threatening with increased rates of infectivity and severity of clinical outcomes.
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